Immobilization of Proteins of Cell Extract to Hydrogel Networks Enhances the Longevity of Cell-Free Protein Synthesis and Supports Gene Networks

Xiaofei Ouyang, Xiaoyu Zhou, Sze Nga Lai, Qi Liu, Bo Zheng

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Herein, we constructed a new type of hydrogel based artificial cells supporting long-lived protein synthesis, post-translational modification, and gene networks. We constructed the artificial cells by immobilizing the transcription and translation system from E. coli cytoplasmic extract onto the polyacrylamide hydrogel. With the continuous supply of energy and nutrition, the artificial cells were capable of stable protein expression for at least 30 days. Functional proteins which were difficult to produce in vivo, including colicin E1 and urokinase, were synthesized in the artificial cells with high bioactivity. Furthermore, we constructed a sigma factor based genetic oscillator in the artificial cells. The artificial cells not only provide a powerful platform for continuous protein synthesis and convenient design and testing of genetic networks, but also hold great promise for the development of metabolic engineering, drug delivery, and biosensors.

Original languageEnglish
Pages (from-to)749-755
Number of pages7
JournalACS Synthetic Biology
Volume10
Issue number4
DOIs
Publication statusPublished - 16 Apr 2021

Keywords

  • cell-free gene expression
  • genetic oscillation
  • microfluidics
  • post-translational modifications
  • synthetic cell

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