Liver cancer detection by a simple, inexpensive and effective immunosensor with zinc oxide nanoparticles

Congo Tak Shing Ching, Nguyen van Hieu, Teng Yun Cheng, Lin Shien Fu, Tai Ping Sun, Ming Yen Liu, Su Hua Huang, Yan Dong Yao

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


Regular monitoring of blood α-fetoprotein (AFP) and/or carcino-embryonic antigen (CEA) levels is important for the routine screening of liver cancer. However, AFP and CEA have a much lower specificity than des-γ-carboxyprothrombin (DCP) to detect liver cancer. Therefore, the study reported here was designed, to develop a screen-printed DCP immunosensor incorporating zinc oxide nanoparticles, for accurate determination of DCP. The designed immunosensor shows low detection limits for the detection of DCP: 0.440 ng/mL (based on impedance measurement), 0.081 ng/mL (based on real part of impedance measurement) and 0.078 ng/mL (based on imaginary part of impedance measurement), within the range of 3.125 ng/mL to 2000 ng/mL. In addition, there was little interference to DCP determination by molecules such as Na+, K+, Ca2+, Cl−, glucose, urea, and uric acid. It is therefore concluded that the DCP immunosensor developed and reported here is simple, inexpensive and effective, and shows promise in the rapid screening of early-stage liver cancer at home with a point-of-care approach.

Original languageEnglish
Pages (from-to)29408-29418
Number of pages11
JournalSensors (Switzerland)
Issue number11
Publication statusPublished - 20 Nov 2015


  • DCP
  • Immunosensor
  • Liver cancer
  • Nanoparticle
  • Screening


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