Potential tumor-suppressive role of monoglyceride lipase in human colorectal cancer

H. Sun, L. Jiang, X. Luo, W. Jin, Q. He, J. An, K. Lui, J. Shi, R. Rong, W. Su, C. Lucchesi, Y. Liu, M. S. Sheikh, Y. Huang

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

Human monoglyceride lipase (MGL) is a recently identified lipase and very little is known about its regulation and function in cellular regulatory processes, particularly in context to human malignancy. In this study, we investigated the regulation and function of MGL in human cancer(s) and report that MGL expression was either absent or reduced in the majority of primary colorectal cancers. Immunohistochemical studies showed that reduction of MGL expression in the colorectal tumor tissues predominantly occurred in the cancerous epithelial cells. MGL was found to reside in the core surface of a cellular organelle named 'lipid body'. Furthermore, it was found to interact selectively with a number of phospholipids, including phosphatidic acid and phosphoinositol(3,4,5)P3, phosphoinositol(3,5)P2, phosphoinositol(3,4)P2 and several other phosphoinositides, and among all phosphoinositides analyzed, its interaction with PI(3,4,5)P3 was found to be the strongest. In addition, overexpression of MGL suppressed colony formation in tumor cell lines and knockdown of MGL resulted in increased Akt phosphorylation. Taken together, our results suggest that MGL plays a negative regulatory role in phosphatidylinositol-3 kinase/Akt signaling and tumor cell growth.

Original languageEnglish
Pages (from-to)234-241
Number of pages8
JournalOncogene
Volume32
Issue number2
DOIs
Publication statusPublished - 10 Jan 2013

Keywords

  • Akt
  • colorectal cancer
  • gene expression
  • monoglyceride lipase
  • phosphatidylinositides

Fingerprint

Dive into the research topics of 'Potential tumor-suppressive role of monoglyceride lipase in human colorectal cancer'. Together they form a unique fingerprint.

Cite this